AFM Study of Structural Changes in Supported Planar DPPC Bilayers Containing General Anesthetic Isofluorane (Plus Additional AFM Experiments)

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Permanent URL	http://hdl.lib.byu.edu/1877/2172
Title	AFM Study of Structural Changes in Supported Planar DPPC Bilayers Containing General Anesthetic Isofluorane (Plus Additional AFM Experiments)
BYU Creator	Busath, David D. Conley, Hiram McKell, Chad
Keywords	atomic force microscopy; AFM; planar bilayers; anesthesia; DPPC
Description/Abstract	Atomic force microscopy (AFM) is a remarkable tool for assessing the structural properties of supported lipid planar bilayers under different physiological conditions. Previous work has shown that incorporation of anesthetics into artificial lipid bilayers results in domain formation [1], destruction of lipid aggregates and patches [2], anesthetic-lipid mixed micelle formation [2], and the development of interdigitated phases of reduced thickness compared to anesthetic-free bilayers [3]. In particular, these interdigitated phases are suspected to affect the structure and activity of membrane proteins, such as ion channels, and thus further research with proteinembedded bilayers exposed to anesthetics could reveal the mechanism responsible for disrupting action potentials. In this study, we inspect the effect of the general anesthetic isofluorane, a drug used widely by physicians to induce anesthesia in patients, on supported dipalmitoylphophatidylcholine (DPPC) planar bilayers using AFM. Bilayers were formed using the vesicle fusion method and imaged with Pico AFM.
Source	D. D. Busath, C. Hiram, M. Chad. (2010). AFM study of structural changes in supported planar DPPC bilayers containing general anesthetic isofluorane (plus additional AFM experiments).;
Date Original	2010
Date Digital	2010-03-30
Language	English eng en
Publication Type	Articles
Type	Text
Format	application/pdf
Owning Institution	Brigham Young University
BYU College	College of Life Sciences
BYU Department	Physiology and Developmental Biology
Copyright Status	(c) 2010 Chad McKell, et al.;
Copyright Use Information	http://www.lib.byu.edu/generic_copyright.html

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AFM Study of Structural Changes in Supported Planar DPPC Bilayers Containing General Anesthetic Isofluorane (Plus Additional AFM Experiments)

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